【摘要】 目的:分析男性精液参数与卵裂率、优质胚胎率及妊娠率的相关性,探讨体外受精-胚胎移植(IVF-ET)周期中精子形态对精子功能的影响。方法:分析82个受精率100%的IVF-ET周期。以Diff-Quickstain方法染色,密度梯度离心法处理精液,WHO精子形态学标准评估精子形态,精子正常形态率≥14%为正常组,<14%者为异常组。观察精液处理前后精子参数变化,比较妊娠组与未妊娠组的年龄、移植日子宫内膜厚度和精液各参数的情况。比较精液处理前后精子正常形态率与两组的正常受精率、卵裂率、优质胚胎率和妊娠率。结果:①密度梯度离心法处理后82例精液密度明显降低,活动力明显提高,差别有统计学意义。②精液处理前精子正常形态率:正常组11例(13.41%),异常组71例(86.59%);处理后正常组34例(41.46%),异常组48例(58.53%)。异常组精液处理后正常精子形态的比例提高,中位数(P25,P75)分别为8.15%(6.38%,10.50%)vs.12.41%(9.98%,18.58%),差别有统计学意义(P<0.01)。③精液处理前精子正常形态率正常组和异常组的密度和活动... 更多还原

【Abstract】 Objective:To Analyze the relation between the sperm parameters and cleavage rate,the quality of embryos and the pregnancy rate,investigate the impact of sperm morphology on spermatozoal functions in in-vitrofertilization.Methods:We only included couples whose fertilization rate was 100%.A total of 82 cycles were included in our study.Before and after density gradient centrifugation,sperm morphology was evaluated by Diff-Quik stain according to the WHO criterion.The normal proportion of normal mo... 更多还原