【作者】 刘曼曼; 余涛; 陈旻; 侯风刚; 罗梅宏; 徐娟萍;
【Author】 LIU Man-man1,YU Tao1,CHEN Min1,HOU Feng-gang1,LUO Mei-hong2,XU Juan-ping2(1.Department of Oncology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China;2.Department of Central Laboratory,Shanghi Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)
【机构】 上海中医药大学附属上海市中医医院肿瘤科; 上海中医药大学附属上海市中医医院中心实验室;
【摘要】 目的探讨抑癌方对小鼠结肠癌移植瘤微血管(microvessel,MV)生成和血管生成拟态(vasculogen-ic mimicry,VM)形成的影响。方法用CT26结肠癌细胞建立小鼠结肠癌移植瘤模型,分生理盐水(nor-mal saline,NS)组,抑癌方高、中、低剂量组及化疗药组,分别灌服NS,抑癌方高、中、低剂量及腹腔注射5-氟脲嘧啶(5-fluorouracil,5-Fu)。处理后比较各组之间瘤体质量;采用血管内皮细胞CD34免疫组织化学法和CD34免疫组织化学法+糖原蛋白过碘酸希夫氏双染法分别检测瘤组织中微血管密度(microvessel density,MVD)及血管生成拟态密度(vasculogenic mimicry density,VMD)。结果与NS组比较,抑癌方各剂量组及化疗组瘤体质量均显著降低(P<0.05);但抑癌方各组瘤体质量均显著高于化疗组(P<0.05)。NS组癌巢间MV和VM丰富,与之相比较,抑癌方各剂量组和化疗组瘤组织中MVD和VMD均显著减少(P<0.05)。结论抑癌方对小鼠移植瘤有一定的抑制作用,能够抑制结肠癌移植瘤中MV和V... 更多还原
【Abstract】 Objective To research the effects of Yiaifang(YAF),a traditional Chinese medicine prescription,on microvessels(MV) and vasculogenic mimicry(VM) formation in mice bearing transplanted colon cancer.Methods The model of transplanted colon cancer was induced by colon cancer cell CT26 in mice.All mice were randomly divided into normal saline(NS) treated group,high-,medium-and low-dose YAF treated groups and chemotherapy group.Mice in NS treated group were fed with NS;while mice in the high-,medium-,l... 更多还原
【基金】 上海市自然基金(10ZR1428700);;上海市中医肿瘤特色专科建设项目(SHDCI2007504);;上海市中医肿瘤优势专科建设项目(2008YSZK008)
中华医学之家:http://www.xinxi85.com
投稿信箱:zhyxzj85@163.com
联系电话:029--85327298 主编QQ:693891972