细胞与分子免疫学杂志 , Chinese Journal of Cellular and Molecular Immunology, 编辑部邮箱 , 2011年10期 【作者】 刘小友; 徐健;
【Author】 LIU Xiao-you1, XU Jian2* 1Department of Organ Transplantation, Zhujiang Hospital, Guangzhou 510282; 2Department of Organ Transplantation, Nanfang Hospital, Guangzhou 510515, China
【机构】 南方医科大学珠江医院器官移植科; 南方医科大学南方医院器官移植科;
【摘要】 目的:探讨小RNA-223(miR-223)在肾移植后急性排斥反应(AR)中的作用。方法:对近一年我院33例接受肾移植手术的患者进行常规检查,每周1次,以判断移植肾的排斥情况,同时定期分离外周血细胞用PCR检查miR-223。另外,抽取10名健康志愿者的外周血,并分离外周血单个核细胞(PBMCs),经植物血凝素(PHA)激活处理后,检查miR-223表达的变化。结果:发生AR患者的外周血中,miR-223的表达水平出现2倍以上的升高。经PHA刺激激活的PB-MCs中miR-223的表达水平与未经PHA激活PBMCs相比较,升高3.76倍。此外,以miR-223来预测AR,可以得到92%的灵敏度和90%的特异性。结论:肾移植AR可能伴随着外周血miR-223表达的升高,提示miR-223可能在肾移植AR中发挥一定的作用。 更多还原
【Abstract】 AIM: To explore the role of miR-223 in the acute rejection after kidney transplantation. METHODS: 33 patients who received kidney transplantation in our hospital within a year were collected and 12 of them appeared acute rejection within 1 month after surgery. In all the patients, the peripheral blood miR-223 level was collected and detected by blind arrangements. Furthermore, PBMCs from healthy volunteers were also collected and stimulated by PHA and then miR-223 level was measured. RESULTS: In... 更多还原 【关键词】 miR-223; 肾移植; 急性排斥反应; 【Key words】 miR-223; kidney transplantation; acute rejection; 【基金】 广东高校优秀青年创新人才培养计划资助项目(LYM09041);广东省自然科学博士启动基金资助项目(06301161)【分类号】R699.2
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