| 王文军1,2，唐罗生3，杨新光1,2 不同生长期晶状体蛋白对体外培养的大鼠视网膜神经节细胞存活的促进作用 |
| 论文编辑部-新丝路理论网 2011-03-31 15:30:38 作者:站长 来源: 文字大小:[大][中][小] |
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Title:
The effect of developing crystallin on survival and growth of rat RGCs in vitro
- 文章编号:
- 1671-8259(2011)02-0175-05
- 作者:
- 王文军1; 2; 唐罗生3; 杨新光1; 2
- 1. 西安交通大学医学院附属广仁医院眼科，陕西西安 710061；2. 西安市第四医院眼科，陕西西安 710004；3.中南大学湘雅二医院眼科，湖南长沙 410011
- Author(s):
- WANG Wen-jun1; 2; TANG Luo-sheng3; YANG Xin-guang1; 2
- 1. Department of Ophthalmology, Affiliated Guangren Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061; 2. Department of Ophthalmology, Xi’an No.4 Hospital, Xi’an 710004; 3. Department of Ophthalmology, Xiangya Second Hospital of Central South University, Changsha 410011, China
- 关键词:
- ; 晶状体蛋白; 视网膜神经节细胞; 细胞凋亡; 大鼠; 细胞培养
- Keywords:
- ; crystallin; retinal ganglion cell; apoptosis; rat; cell culture
- 分类号:
- R774.6
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 摘要：目的 观察不同生长期大鼠的晶状体蛋白对体外培养的大鼠视网膜神经节细胞(retinal ganglion cells, RGCs)是否具有不同的保护作用。方法 分别提取出生后1d、2周、6月、1年期的SD大鼠晶状体可溶性蛋白，加入培养的RGCs中，观察其对RGCs存活时间、突触生长状况及凋亡的影响。结果 同期提取的晶状体蛋白各不同浓度作用于RGCs，促进其存活的作用有差异，最佳有效浓度为10-5g/L。不同生长期大鼠的晶状体蛋白可以延长RGCs存活时间，并随生长期增长而延长，但1d组与2周组无显著性差异。加入晶状体蛋白后RGCs凋亡减少，随生长期增长而逐级减少。
结论 晶状体可溶性蛋白可显著促进离体培养的RGCs的存活和生长，晶状体蛋白的最佳有效质量浓度为10-5g/L。不同生长期晶状体蛋白的神经保护作用有所不同，随生长期延长，作用逐渐增强。
- Abstract:
- ABSTRACT: Objective To study whether crystallin of rats at different growth stages has different protective effects on survival and growth of rat retinal ganglion cells (RGCs) in vitro. Methods RGCs in SD rats were cultured in DMEM/F12 containing crystallin of different age rats (1 day, 2 weeks, 6 months and 1 year). The RGCs survival time, process growth and apoptosis were observed. Results The best concentration of soluble crystallin was 10-5g/L after comparing three different concentrations. Crystallin of different growth stage rats could prolong RGCs’ survival time, which increased with the increase of growth period. However, 1d group and 2w group did not differ significantly. RGCs’ apoptosis decreased after crystallin was added and it gradually decreased with prolonged growth period. Conclusion Crystallin can significantly promote the survival and growth of rat RGCs in vitro. And crystallin of 10-5g/L is the most effective concentration among various concentrations. Crystallin of different growth stages has a different neuroprotective effect, which is enhanced with the extension of growth period.
参考文献/References
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基金项目：中南大学研究生学位论文创新基金资助
Supported by the Graduate Degree Thesis Innovation Foundation of Central South University
通讯作者：唐罗生，教授. E-mail: tangls57@gmail.com
作者简介：王文军(1975-)，男(汉族)，医学博士. 研究方向：玻璃体视网膜疾病的基础与临床研究.E-mail: wangwjchina@sohu.com |
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