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张咏梅1,张雪玉2,刘莉莉1,吴 蔚1,马文娟1实时荧光定量PCR检测宫颈癌组织中HGF mRNA和c-Met mRNA的表达
论文编辑部-新丝路理论网   2011-03-31 15:55:17 作者:中华医学之家:http://www.xinxi85.com 来源: 文字大小:[][][]
Title:
 Detection of expressions of HGF mRNA and c-Met mRNA in cervical carcinoma with QRT-PCR method
文章编号:
1671-8259(2011)02-0234-04
作者:
 张咏梅1张雪玉2刘莉莉1吴 蔚1马文娟1
 宁夏医科大学:1. 研究生学院;2. 附属医院妇科,宁夏银川 750004
Author(s):
 ZHANG Yong-mei1 ZHANG Xue-yu2 LIU Li-li1 WU Wei1 MA Wen-juan1
 1. Graduate School; 2. Gynecology Department of the Affiliated Hospital, Ningxia Medical University, Yinchuan 750004, China
关键词:
 宫颈癌肝细胞生长因子原癌基因c-Met实时荧光定量PCR
Keywords:
 cervical carcinoma hepatocyte growth factor (HGF) c-Met real-time quantitative reverse transcription-polymerase chain reaction (QRT-PCR)
分类号:
R711.74
DOI:
-
文献标识码:
A
摘要:
 摘要:目的   检测宫颈癌组织中肝细胞生长因子(HGF)及其受体原癌基因c-Met的分子表达水平,并探讨其临床意义。方法   采用实时荧光定量PCR(QRT-PCR)技术检测43例宫颈癌,30例宫颈上皮内瘤变Ⅲ和27例正常宫颈组织标本中HGF和c-Met的分子表达水平。结果 HGF mRNA和c-Met mRNA在宫颈癌组织中均呈高表达,其与宫颈癌患者临床分期、肿瘤直径大小、浸润深度、淋巴结转移有关(P均<0.05),而与肿瘤的分化程度无关(P>0.05)。HGF mRNA和c-Met mRNA之间存在正相关(r=0.666,P<0.01)。结论 QRT-PCR技术可对宫颈癌组织中的HGF mRNA和c-Met mRNA表达进行定量检测;HGF mRNA和c-Met mRNA的高表达与宫颈癌的侵袭转移有关。两者联合监测有利于早期诊断宫颈癌,为宫颈癌的治疗和预后提供参考。
Abstract:
 ABSTRACT: Objective   To investigate the expressions of hepatocyte growth factor (HGF) and its receptor proto-oncogene c-Met in cervical carcinoma and their clinical significance. Methods  The expressions of HGF mRNA and c-Met mRNA were detected by real-time quantitative reverse transcription-polymerase chain reaction (QRT-PCR) method in 43 cervical carcinoma tissues, 30 cervical intraepithelial neoplasm (CIN) Ⅲ tissues and 27 normal cervical tissues. Results   The positive expressions of HGF mRNA and c-Met mRNA increased remarkably in cervical carcinoma tissues (P<0.05). In cervical carcinoma, the expressions of HGF mRNA and c-Met mRNA were correlated with the clinical stage, tumor size, invasion of deep muscular layer and lymph node metastasis (P<0.05), but not with the grade of tumor pathology (P>0.05). There was a significant positive correlation between HGF and c-Met in cervical carcinoma (r=0.666, P<0.01). Conclusion   QRT-PCR used to detect the expressions of HGF mRNA and c-Met mRNA may be an accurate and reliable means for early diagnosis of cervical carcinoma. Over-expression of HGF and c-Met mRNA may both be involved in the invasion and metastasis of cervical carcinoma. Monitoring HGF mRNA and c-Met mRNA would facilitate the treatment and prognosis of cervical carcinoma.

参考文献/References

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基金项目:宁夏自然科学基金资助项目(No.NZ08104)
Supported by the Natural Science Foundation of Ningxia (No.NZ08104)
通讯作者:张雪玉,教授,硕士生导师. E-mail: zhxueyu@163.com
作者简介:张咏梅(1976-),女(汉族),在读硕士,主治医师. 研究方向:妇科肿瘤. E-mail: zhangyongmei008@163.com

华医学之家:http://www.xinxi85.com

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