高脂诱导肥胖小鼠视网膜变性的形态学改变 Morphological changes of retinal degeneration in obese mice fed with high-fat diet推荐 CAJ下载 PDF下载 不支持迅雷等下载工具,请取消加速工具后下载。 解剖科学进展 , Progress of Anatomical Sciences, 编辑部邮箱 , 2011年05期 【作者】 白霞; 胡庆哲; 马玉东; 施蓓; 孟凡鑫;
【Author】 BAI Xia1, HU Qing-zhe1, MA Yu-dong2, SHI Bei1, MENG Fan-xin1 (1. Department of Physiology, College of Basic Medical Sciences, China Medical University, Liaoning Shenyang 110001 ; 2. Department of ICU, Fengtian Hospital Affiliated to Shenyang Medical College, Liaoning Shenyang 110024 China)
【机构】 中国医科大学基础医学院生理学教研室; 沈阳医学院奉天医院ICU科;
【摘要】 目的探讨高脂饮食建立的C57BL/6小鼠肥胖模型的视网膜变性的超微结构改变。方法高脂饲料喂养19周后,小鼠分为肥胖抵抗(DIO-R)组和肥胖倾向(DIO)组,同时对照组小鼠给予基础饲料喂养。应用光镜,透射电镜及TUNEL法检测三组小鼠视网膜超微结构的改变及凋亡情况。结果与对照组及DIO-R组比较,DIO组小鼠视网膜及外核层(ONL)变薄,感光细胞出现凋亡及坏死,其外段视盘排列紊乱,视盘膜溶解、断裂,视网膜神经节细胞体积变小,细胞质分布紊乱,核染色质固缩。TUNEL法检测结果显示,在ONL层可见较多的凋亡细胞体,DIO组细胞凋亡率显著高于对照组和DIO-R组。结论高脂诱导的肥胖可能导致C57BL/6小鼠视网膜变性及细胞凋亡。 更多还原
【Abstract】 Objective To investigate the ultrostructural changes of retina caused by high-fat diet induced obesity in C57BL/6 mice. Methods Mice were fed a high-fat diet for 19 weeks and then divided into diet induced obesity-resistant (DIO-R) group and diet induced obesity (DIO) group, mice in control group were fed with a basal diet at the same time. The retinal ultrostructural changes were detected by light and transmission electron microscope, the cellular apoptosis was detected by TUNEL. Results The re... 更多还原 【关键词】 肥胖; 视网膜变性; 凋亡; C57BL/6小鼠; 【Key words】 obesity; retinal degeneration; apoptosis; C57BL/6 mice; 【基金】 辽宁省教育厅基金(No.L2010558)【分类号】R774.1
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