重组CRT/MAGE-A3腺病毒转染SGC-7901细胞及对其抑制作用的研究 The inhibition effect of transfected Ad-CRT/MAGE-A3 on SGC-7901 cells推荐 CAJ下载 PDF下载 不支持迅雷等下载工具,请取消加速工具后下载。
解剖科学进展 , Progress of Anatomical Sciences, 编辑部邮箱 , 2011年05期 【作者】 刘新莉; 赵丹; 李岩; 马萍;
【Author】 LIU Xin-li, ZHAO Dan, LI Yan, MA Ping (Second Laboratory of Tumor Research Institute, First Affiliated Hospital, China Medical University, Shenyang 110001 China)
【机构】 中国医科大学附属第一医院肿瘤研究所第二研究室;
【摘要】 目的探讨同时携带CRT基因及MAGE-A3基因的重组腺病毒载体转染人胃癌细胞SGC-7901的表达及对其体外侵袭、凋亡及血管形成能力的影响。方法 Ad-CRT/MAGE-A3转染SGC-7901,转染48h后以Western blotting法检测CRT及MAGE-A3蛋白的表达,Transwell方法检测Ad-CRT/MAGE-A3对SGC-7901细胞侵袭的影响,AnnexinⅤ-FITC/PI双染法检测Ad-CRT/MAGE-A3对SGC-7901细胞凋亡的影响,小管形成实验检测Ad-CRT/MAGE-A3对血管形成的影响。结果转染48h,Western blotting结果显示转染Ad-CRT/MAGE-A3的SGC-7901细胞能够表达CRT及MAGE-A3蛋白,Transwell检测结果显示Ad-CRT/MAGE-A3能够明显抑制SGC-7901的侵袭能力,AnnexinⅤ-FITC/PI双染法检测结果证实Ad-CRT/MAGE-A3在实验的转染条件下对SGC-7901并无诱导凋亡的作用,小管形成实验结果显示Ad-CRT/MAGE-A3能够明显抑制血管形成。结论 Ad-CR... 更多还原
【Abstract】 Objective To explore the expressions of CRT and MAGE-A3 genes and the inhibition effect of Ad-CRT/MAGE-A3 on the invasion, apoptosis and anti-angiogenesis of SGC-7901. Methods An adenovirus expression vector for CRT/MAGE-A3 (Ad-CRT/MAGE-A3) was transfected into SGC-7901. Forty-eight hours after transfection, Western blotting assay was adopted to identify the expression of CRT and MAGE-A3, anti-invasion was observed by transwell assay, apoptosis was assayed with AnnexinⅤ-FITC/PI doubled stained f... 更多还原 【关键词】 CRT; MAGE-A3; 重组腺病毒载体; 细胞侵袭; 血管形成; 胃癌; 【Key words】 CRT; MAGE-A3; invasion; angiogenesis; gastric carcinoma; 【基金】 辽宁省自然科学基金资助项目(No.20042073);辽宁省科技计划项目(2009225008-1)No【分类号】R735.2
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