【摘要】目的：采用细菌药敏实验中通常采用的微量稀释法并添加细胞生长指示剂检测临床分离株对异烟肼、利福平、硫酸链霉素、乙胺丁醇耐药性，并与比例法结果作比较，确定四种抗生素的临界浓度。方法：采用微量稀释显色法检测63株临床分离结核分支杆菌的最小抑制浓度，并将结果与比例法结果进行配对比较分析，从而判断结核分支杆菌是否耐药。结果：用卡方配对比较确定了抗结核药物异烟肼的临界浓度为0.25μg/mL、利福平为1μg/mL、硫酸链霉素8μg/mL、乙胺丁醇4μg/mL。结论：用微量稀释显色法检测结核分枝杆菌的耐药性和比例法比较检测结果有较好的一致性，可以用于临床结核分枝杆菌药物敏感性检测。

　　【关键词】结核分支杆菌；微量稀释显色法；耐药性

　　Microdilution Colorimetric method Determinated Antibiotic Susceptibility of Mycobacterium tuberculosis

　　【Abstract】objiectTo test the susceptibility of bacteria commonly used in micro-dilution method and add the cell groth indicator testing clinical isolated，

　　detection of clinical isolates of four kinds of antibiotics，isoniazid，rifampicin，streptomycin sulfate，ethambutol resistance，and with the proportion method results are compared to determine the critical concentration of the four kinds of antibiotics

　　MthodsMicrodilution Colorimetric method assay 63 clinical isolates of Mycobacterium tuberculosis minimum inhibitory concentration，and with the proportion method results are matched a comparative analysis in order to determine whether the drug-resistant Mycobacterium tuberculosis.results Compared with the chi-square paired set of critical anti-TB drugs isoniazid concentration 0.25μg/mL，rifampicin for 1μg/mL，streptomycin sulfate 8μg/mL，ethambutol 4μg/mL . Conclusion Using Microdilution Colorimetric method detection of drug resistance of Mycobacterium tuberculosis compared with the proportion method has good consistency.

　　【Key words】Mycobacterium tuberculosismicrodilution Colorimetric method drug resistance

　　近年来，耐多药结核菌和广泛耐药结核菌的存在已经严重威胁着人类的健康，一线药物已经不能满足临床治疗的需要，世界卫生组织就如何管理耐药结核病提供最新的指导和建议[1]，以便最大限度的抑制耐药结核病的传播，如何快速检测此类结核菌，已经成为临床检验的一大难题。（剩余1485字）

中华医学之家：http://www.xinxi85.com

投稿信箱：zhyxzj85@163.com

联系电话：029--85327298     主编QQ：693891972